Plasmid

Part:BBa_K4630119:Design

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-12)


pET28a-eGFP-stgframe


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1154
    Illegal SpeI site found at 912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 912
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1220
    Illegal BamHI site found at 903
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1154
    Illegal SpeI site found at 912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1154
    Illegal SpeI site found at 912
    Illegal NgoMIV site found at 1252
    Illegal NgoMIV site found at 2840
    Illegal NgoMIV site found at 3000
    Illegal NgoMIV site found at 6051
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3920


Design Notes

We set BsaI recognition sites upstream the stgRNA scaffold, so we could determine the efficiency of different stgRNA.


Source

Complete synthesis and subcloning.

References